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    Genomics Shared Resource

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    Please remember to cite the Shared Resources!

    Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.

    We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)

    About Us(back to top)

    The Genomics Shared Resource (GSR) provides a centralized, comprehensive resource of high-throughput genomics technologies to OSUCCC – James researchers and non-CCC members.

    The GSR offers instrumentation and expertise for DNA and RNA analysis using sequencing, genotyping, real-time PCR, Affymetrix GeneChips, nCounter Analysis, next-generation sequencing library generation, next-generation sequencing (through partner laboratories), nucleic acid extraction, and QC for RNA/DNA/proteins.

    OSUCCC member and non-member investigators have unlimited access to training, consultation, troubleshooting and assistance in experimental design.

    Biomedical Research Tower
    460 W. 12th Ave.
    Columbus, OH 43210

    Phone: 614-247-8185
    Amanda.Toland@osumc.edu

    Availability: Monday-Friday, 8:30 a.m.-5 p.m. (or by appointment)

     

    • Quantitative Real-Time PCR/Gene Expression/NanoString
      • 236/240 Biomedical Research Tower
        460 W. 12th Ave.
        Columbus, OH 43210
        Phone: 614-688-5810

        Paolo.Fadda@osumc.edu 

     

     

    Meet the Team(back to top)

    Amanda Ewart Toland, PhD, FACMG - Director amanda.toldand@osumc.edu

    Amanda is a professor with a joint appointment in the Department of Cancer Biology and Genetics and the Department of Internal Medicine, Division of Human Genetics. She has board certification through the American Board of Genetics and Genomics in PhD Medical Genetics and Clinical Molecular Genetics. Her research focuses on the genetics of cancer susceptibility and modifiers of cancer risk in the general population and in high-risk families, genetics of cutaneous squamous cell carcinomas and the interplay between germline genetic variation and somatic events in tumors. Dr. Toland has extensive experience in different genotyping platforms and has used next-generation sequencing (including exome-sequencing, targeted sequencing and RNA-seq), NanoString technologies, and qPCR extensively for her own research. She can be reached at amanda.toldand@osumc.edu

    Pearlly Yan, PhD - Technical Director pearlly.yan@osumc.edu

    Pearlly is a research associate professor at The Ohio State University College of Medicine, Division of Hematology. She has been with the GSR since 2008 and was responsible for setting up the GSR – Illumina NGS services. Dr. Yan collaborates with computation scientists in the development of custom scripts for novel global analyses. For instance, Dr. Yan optimized cell-based ultralow input mRNA-seq with GSR team members and research collaborators. With her R50 Research Specialist Award (NCI) support, she and her computation colleague and trainees optimize transcriptome profiling from as low as 50 cells resulting in one computation publications and six research papers. In 2021, Dr. Yan collaborated with Novartis Institutes of Biomedical Research using the cell-based mRNA-seq on a dose escalation- and a dose expansion trial. The work resulted in a 2022 AACR Annual Meeting platform presentation and a 2023 manuscript submission. This cutting-edge approach is now a standard GSR service and is embraced by many research groups across the OSU campus. Dr. Yan works closely with Drs. Toland and Chen on developing new single cell services, interacts with new users and users starting new NGS projects and oversees the day-to-day operations of the NGS services.

    Xi Chen, PhD - Research Associate II xi.chen@osumc.edu

    Xi received his PhD in Biochemistry and Molecular Biology from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. With over 15 years of research experience in molecular biology, cell biology, and cancer biology, Dr. Chen became a part of the Genomics Shared Resource team in 2016. He brought with him a wealth of expertise in molecular biology, cell biology, and cancer biology. Within his GSR role, his responsibilities encompass NGS application development, protocol optimization, and the creation of diverse sequencing libraries such as RNA-seq, DNA-seq, Exome analysis, Targeted-panel sequencing, and Reduced Representation Bisulfite Sequencing. Recently, Dr. Chen has successfully integrated two single-cell sequencing methods, namely SMART-seq2 and 10X Genomics single-cell gene expression, into his work. Moreover, he is currently engaged in the development of additional 10X Genomics single-cell analyses, including single-cell RNA profiling from fixed tissue and spatial gene expression.

    Christine Daugherty, MS - Research Specialist christine.daughtery@osumc.edu

    Christine earned her master’s degree in Molecular Genetics from the Ohio State University. She has more than 24 years of experience in molecular biology, sequencing and genotyping research and analysis. Her expertise includes Sanger-based DNA sequencing and genotyping (including cell line verification and SHAPE) on ABI PRISM 3730XL DNA Analyzers.

    Paolo Fadda, PharmD - Senior Research Scientist paolo.fadda@osumc.edu

    Paolo earned a PharmD degree from Sassari University (Italy) and brings more than 3 years of research experience in molecular biology and more than thirteen years in the shared resource. Dr. Fadda has experience in nucleic acids isolation and QC, gene expression analysis (including TaqMan gene expression assays), TaqMan Low Density Array (TLDA), TaqMan microRNA assays, SYBR Green chemistry and data analysis, genotyping analysis (SNP and CNV), Fluidigm genotyping, copy number assessment and gene expression, Digital PCR using BioRad QX200 droplet reader, spatial transcriptomics and proteomics using the NanoString GeoMx Digital Spatial profiler, generate and QC NGS libraries for NanoString GeoMx Digital Spatial profiler samples, high-throughput gene expression analysis using the nCounter System from NanoString Technologies and Affymetrix Technology applications.

    Estela Puchulu-Campanella, MS - Research Associate estela.puchulu-campanella@osumc.edu

    Estela has more than 30 years of research experience in academia and industry settings. Her research focus includes protein and nucleic acid extraction and analysis, next-generation sequencing technology, hematology, immunohistochemistry, immunofluorescence, flow cytometry, micro computed tomography, microscopy, electrophoresis and chromatography. In the GSR, she generated NGS libraries including RNA-seq (both mRNA and total RNA-seq), targeted NGS panels. She also isolated RNA from fixed tissues using the Covaris E220.

    Huabao Wang, BS - Senior Research Associate, huabao.wang@osumc.edu

    Huabao has over 12 years of experience in preparing sequencing libraries including whole transcriptome, miRNA and targeted genomic DNA libraries. She previously oversaw our Affymetrix microarray offerings as well as DNA/RNA quality control. Currently Huabao is part of our next-generation sequencing team and generates a variety of NGS library types from multiple nucleic acid sources.

    Richard Wiemels, MS - Research Associate II richard.wiemels@osumc.edu

    Richard, earned his master’s from Ohio University and has over 10 years of experience in molecular biology and biochemistry. He joined our team in 2022. His expertise includes nucleic acid isolation and QC, qPCR, RNA-seq, assay development, and chromatography. He is currently working with the NanoString nCounter system, qPCR, and ddPCR.

     

    Available Services(back to top)

    Go To eRAMP
    • 10X single-cell library preparation (3’, 5’, ATAC-seq, VDJ)
    • NGS library generation (RNA-seq, limiting cell RNA-seq, exome and targeted DNA sequencing, single cell)
    • Spatial Transcriptomics via NanoString GeoMx Digital Spatial Profiling
    • Digital Droplet PCR
    • Quantitative Real-Time PCR (qRT-PCR)
    • Sanger Sequencing
    • SHAPE analyses
    • Human Cell Line Verification
    • Affymetrix Gene Expression Profiling
    • NanoString nCounter digital mRNA and miRNA standard or custom panels
    • DNA and RNA Quality Control and Quantification (Bioanalyzer and TapeStation and Qubit)
    • DNA and RNA isolation
    • NGS sequencing (through partner lab)  

    Click here for full list of services and fees

    How To's (back to top)

    Please remember to cite the Shared Resources!

    Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.

    We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)

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