Proteomics Shared Resource
Please remember to cite the Shared Resources!
Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.
We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)
About Us: (back to top)
The Proteomics Shared Resource (PSR) is a joint venture between the OSUCCC – James and the Campus Chemical Instrumentation Center that supports high-quality cancer research by providing technical expertise and state-of-the-art instrumentation to the OSUCCC and The Ohio State University research community. The PSR provides expert consulting, sample preparation, advanced protein and metabolite identification, characterization and quantification, spatial imaging, native mass spectrometry for the analysis of intact protein complexes services to support and enhance cancer research at Ohio State Cancer Center.
250 Biomedical Research Tower
460 W. 12th Ave.
Columbus, OH 43210
Phone: 614-247-8789 (Lab)
Availability: Monday-Friday, 8 a.m.-5 p.m.
Meet the Team: (back to top)
Director of CCIC: Christopher Hadad, PhD
Associate Director of CCIC MSP: Sophie Harvey, PhD
Technical Director: Liwen Zhang, PhD
Research Scientist: Matthew Bernier, PhD
Research Scientist: Chengyu Gao, PhD
Research Scientist: Joshua Gilbert, PhD
Research Scientist: Gong Wu, PhD
Researcher: Brian Fries, PhD
Senior Business Operations Administrator: Heidi Hamblin, MBA
Services
Click here for full list of services and fees
Metabolomics
Offering state-of-the-art instrumentation and workflows for both untargeted and targeted approaches.
Our facility provides comprehensive metabolomic analysis services using state-of-the-art equipment, including:
• Sample preparation
o We accept a wide range of different samples in varying levels of complexity. We offer a range of sample handling services to properly prepare samples for downstream analysis, including lysis and homogenization, protein precipitation, and extraction.
• Untargeted metabolomics and lipidomics
o We can provide untargeted metabolomic and lipidomic profiling and discern differences between sample groups. We can employ positive or negative mode ionization and different separation methods (reverse phase, HILIC etc) depending on the compounds of interest.
• Targeted compound quantification
o We provide targeted compound quantification using a triple quadrupole mass spectrometer (Agilent 6495D). The use of heavy labeled standards and a calibration curve enables absolute quantification studies to be performed. We can perform these studies using either positive or negative mode ionization, and using a variety of separation methods (reverse phase, HILIC etc), optimzied for your compounds of interest.
State-of-the-Art Instrumentation
Our metabolomics instrumentation includes:
• Agilent 6495D
• Thermo Exploris 480
• Thermo Q Exactive plus
These advanced instruments don't just collect data—they reveal the unique chemical fingerprints within intricate metabolomic samples with exceptional reliability.
Mass Spectrometry
• Accurate Mass Analysis to within 1 ppm (ESI and MALDI)
• Nominal Mass Analysis (ESI and MALDI)
• Structure determination
Proteomics
Offering advanced analysis services and state-of-the-art instrumentation for protein research.
Our facility provides comprehensive proteomic analysis services using state-of-the-art equipment, including:
• Sample preparation
o We accept a wide range of samples from simple proteins to complex tissues and offer all sample handling and protein extraction services to properly prepare samples for downstream analysis. Including cell lysis, protein precipitation, protein quantitation, sample clean-up, digestion, and enrichment.
• Protein identification
o We provide identification(s) for protein or a mixture of proteins using bottom-up proteomics approaches. Samples are enzymatically digested, analyzed with nano LC-MS/MS; using either DDA (data-dependent acquisition) or DIA (data-independent acquisition) approaches. Data are then searched for enabling the proteins to be identified.
• Posttranslational modification analysis
o Common PTM analysis includes phosphorylation, ubiquitination, methylation, acetylation and oxidation. Samples may be enriched prior to digestion and then analyzed by LC-MS/MS to determine the sequence of the peptide and the location of the modification. In addition, we have extensive experience working with researchers to identify and characterize customized post-translational modifications and amino acid mutations.
• Protein quantitation
o We offer several approaches to monitor differentiation in protein expression between samples, including label free approaches (spectral counting), TMT (tandem mass tag), and SILAC (stable isotope labeling with amino acids in cell culture).
• Protein-protein interactions
o We routinely perform bottom-up proteomics on co-immunoprecipitation experiments to identify physiologically relevant protein interactions.
• Intact protein and protein complex analysis
o We also offer the analysis of intact proteins and protein complexes using native mass spectrometry approaches (including online buffer exchange-native MS). Which enables the intact mass to be determined of covalent and non-covalent complexes including protein:protein, protein:RNA/DNA, protein:ligand complexes.
State-of-the-art instrumentation
Our proteomics instrumentation includes:
• Bruker timsTOF Pro
• Thermo Orbitrap Eclipse
• Thermo Orbitrap Fusion
• Thermo Q Exactive plus
• Thermo Q Exactive UHMR (for native MS experiments)
MS Imaging
Visualizing spatial distributions of molecules using state-of-the-art MALDI imaging instrumentation
Our facility provides label free imaging services using state-of-the-art equipment, including:
• Sample preparation
o We offer full sample preparation including embedding, sectioning, and matrix deposition.
• Label free imaging of lipids and other small molecules in tissues
o MS imaging can show localization of molecules within different regions of a sample. Accurate mass analysis, combined with fragmentation and/or ion mobility can provide identification for these molecules.
• Protein imaging
o We offer protein imaging either using protein digests and identifying the peptides, or using chemical probes.
Consultation: PSR personnel provides assistance to OSUCCC investigators with the design and implementation of studies involving mass spectrometry. They collaborate with investigators to plan appropriate mass spectrometric analyses, conduct sample preparation, perform MS runs, and analyze data. Additional support includes discussing results, aiding in grant and manuscript preparation, and providing tours, workshops, and teaching as part of outreach efforts. The PSR offers a unique shared resource that is both cutting-edge and cost-effective for the entire OSUCCC membership.
Consultation: PSR personnel provides assistance to OSUCCC investigators with the design and implementation of studies involving mass spectrometry. They collaborate with investigators to plan appropriate mass spectrometric analyses, conduct sample preparation, perform MS runs, and analyze data. Additional support includes discussing results, aiding in grant and manuscript preparation, and providing tours, workshops, and teaching as part of outreach efforts. The PSR offers a unique shared resource that is both cutting-edge and cost-effective for the entire OSUCCC membership.
Please remember to cite the Shared Resources!
Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.
We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)