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    Gene Editing Shared Resource

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    Please remember to cite the Shared Resources!

    Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.

    We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)

    About Us (back to top)

    GEdSR offers comprehensive support for gene-editing projects using CRISPR/Cas technologies. We assist investigators in planning experiments, selecting optimal strategies, generating gene-edited cell lines, and performing functional analyses to assess the effects of specific genetic modifications. Our services cover all stages of the workflow, from initial design and feasibility assessment to validation of edited cells using live-cell imaging and molecular assays.

    Biomedical Research Tower,
    460 West 12th Avenue
    Columbus, OH 43210
    Lab Location: Room 1040
    Office Location:      Room 1018

     

    Meet the Team(back to top)

    Daniela De Martino, PhD - Technical Director Daniela.DeMartino@osumc.edu

    Daniela De Martino, PhD, is the Technical Director of the Gene Editing Shared Resource at The Ohio State University Comprehensive Cancer Center. In this role, she leads and manages the implementation of advanced gene editing technologies, with a focus on CRISPR-based genome engineering and related molecular approaches. Dr. De Martino partners with investigators to support experimental design, optimize workflows, and ensure the delivery of high-quality gene editing services to the research community. Her leadership enhances the core’s capacity to support innovative research across disciplines.


    Scientific Advisor:     Richard Fishel, PhD

    Available Services (back to top)

    • Gene editing in immortalized and primary cell lines
    • Design and execution of CRISPR knock-out (KO) and knock-in (KI) strategies
    • gRNA cloning and/or delivery as RNP complexes
    • Design of repair templates for small knock-ins and point mutations
    • Generation of vectors and biosensor-expressing cell lines for CRISPR screens
    • Production of large repair templates
    • Generation of bulk or clonal CRISPR-KO/KI cell lines
    • Cell sorting and live-cell imaging of gene-edited cells
    • Cas13-based RNA targeting
    • DNA-free gene editing (base editing and PRIME editing)
    • Design and generation of CRISPR screening libraries
    • CRISPR-based genetic screenings
    • Real-time monitoring of cellular processes using the IncuCyte SX5 Live-Cell Analysis System, including proliferation, apoptosis, migration, differentiation, and reporter activity

    Major equipment

    Resources dedicated to the GEdSR include:

    • Dedicated lab space to produce reagents required for gene-editing projects (e.g., molecular cloning and cell genotyping)
    • Dedicated tissue culture room, including all the standard equipment required for manipulation of primary and established cell lines (2 biosafety cabinets, 2 centrifuges and 1 light microscope)
    • Lonza 4D Core Unit system equipped for optimal high-throughput electroporation (Lonza 96-well Shuttle)
    • EVOS M5000 microscope equipped with multiple light cubes for bright-field and fluorescence microscopy, magnification objectives ranging from 4X to 100X and onstage incubator for live-cell imaging
    • CountessFL automated cell counter
    • Sony MA900 cell sorter, equipped with four lasers and 12 detectors, capable of simultaneous sorting of up to four individual cell populations and single-cell plating. The chip-based system allows the selection of appropriate pressure conditions to maximize purity, yield and cell viability in the sorting process.
    • SX5 Incucyte (Sartorius)

    Click here for full list of services and fees

    Latest News (back to top)

    We are planning to add the following services to our portfolio:

    • Gene editing of Pluripotent Stem Cells
    • Performance of pooled CRISPR screens, which provide the investigators with the unique possibility to simultaneously assess the importance of multiple genes on specific biological functions and pathways

    Please remember to cite the Shared Resources!

    Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.

    We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)

    (back to top)