Comparative Pathology and Digital Imaging Shared Resource
Please remember to cite the Shared Resources!
Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.
We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)
About Us (back to top)
The Comparative Pathology and Digital Imaging Shared Resource (CPDISR) provides expert, affordable, experimental pathology support to OSUCCC – James researchers who use animal models to study human disease.
The CPDISR provides support for preclinical efficacy and toxicity animal studies, as well as translational studies that utilize human tissues procured for research. Comprehensive services provided by the CPDISR include: animal blood and other biofluid analyses; macroscopic and microscopic examinations of full tissue sets from various species of laboratory animals; comprehensive histology services on paraffin-embedded and frozen tissues, encompassing special histochemical stains and immunohistochemical/immunofluorescent labeling optimized for animal and human tissues; slide digitization and quantitative image analysis; and hands-on training and consultation for select techniques. CPDISR comparative pathologists are experts in the normal anatomy and physiology, background age- and strain-/breed-related lesions, infectious pathogens, husbandry practices and experimental models of various animal model species. Lesion recognition and interpretation within individual investigations provides a critical component to research that incorporates animal models.
Services and Locations (Back to top)
- Comparative Pathology and Digital Imaging Shared Resource Main Laboratory
- Histology and Immunohistochemistry/Immunofluorescence Laboratory
- Digital Pathology
- Clinical Pathology Services
- Experimental Design and Data Interpretation
Click here for full list of services and fees
Comparative Pathology and Digital Imaging Shared Resource Main Laboratory (back to top)
Ohio State College of Veterinary Medicine
467/471 Veterinary Medicine Academic Building
1900 Coffey Rd.
Columbus, Ohio 43210
Phone: 614-247-8122
Email: CPDISR@osu.edu
Availability: Monday-Friday
Hours: 8 a.m.-5 p.m.
Available Services
- Necropsy services to evaluate all tissues from your research animal models
- Services start with live mice or rats and result in formal reports from our board-certified comparative pathologists
- Phenotyping services to characterize new mouse models
- Biopsy services to evaluate target organs of interest
- Tissue trimming, sample decalcification, snap freezing
Need assistance scheduling? Please contact us! Samples must be submitted through our online submission system, eRAMP interfaced to eREQUEST, through logging in with your Ohio State login (name.#). Once users receive email notification of fiscal approval, samples can then be delivered to the CPDISR for processing.
Please do not submit live animal or blood samples to the lab without scheduling in advance.
Please contact the CPDISR at 614-247-8122 or CPDISR@cvm.osu.edu with questions regarding our online service requests to coordinate scheduled delivery of animals/samples and prior to submission of live animals and blood samples.
Histology and Immunohistochemistry/Immunofluorescence Laboratory (Back to top)
Ohio State College of Veterinary Medicine
302/312/314 Goss Laboratory
1925 Coffey Rd.
Columbus, Ohio 43210
Phone: 614-292-3327
Email: CPDISR@osu.edu
Availability: Monday-Friday
Hours: 8 a.m.-5 p.m.
Experienced histotechnologists provide complete histology services, including tissue processing, embedding and sectioning of both paraffin-embedded and frozen animal tissues. Routine stains such as hematoxylin and eosin, and an extensive menu of both special histochemical and immunohistochemical stains and labels optimized specifically for use on mouse and other animal tissues, are available and continuously expanded. Such stains facilitate the identification of infectious agents, apoptotic and proliferating cells, angiogenesis, hematopoietic cell lineages and more.
Digital Pathology (Back to top)
310 Goss Laboratory
1925 Coffey Rd.
Columbus, Ohio 43210
Services
- Whole slide scanning, 1x3 and 2x3 slides: available on Leica AT2 or Olympus VS200 Scanners
- Immunofluorescent Whole Slide Scanning: Olympus VS200 Scanner
- 60x Dry and 100x Oil Whole Slide Scanning: Olympus VS200 Scanner
Clinical Pathology Services (Back to top)
Services include hematology (complete blood counts with white blood cell differentials), serum biochemical panels, analysis of urine and other body fluids and cytology.
Qualified laboratory professionals trained in veterinary laboratory techniques perform all tests. The laboratory staff is committed to research in improved diagnostics, reference range development and state-of-the art assays. New tests are continually added as experimental needs change. A network of reference laboratories is available for tests not performed in-house.
The CPDISR participates in quarterly quality assurance testing for hematology and chemistry of laboratory animal samples in addition to the urinalysis and cytology modules through the Veterinary Laboratory Association.
Experimental Design and Data Interpretation (Back to top)
Comparative pathologists actively participate in experimental design prior to submitting animals/tissues for pathologic evaluation as well as for subsequent studies and data interpretation. Recommendations are given for various ancillary tests (i.e. more specialized clinical pathology testing, electron microscopy, imaging, behavioral or physiological testing) provided by other shared resources or Ohio State research laboratories to further elucidate identified morphologic lesions or to rule out functional abnormalities when no morphologic lesions are found.
Routine contributions are also made to grant applications, manuscripts and oral/poster presentations in the form of digital photographs of macroscopic and microscopic lesions, details about methods and pathologic findings, interpretation of results and overall critical editorial review. Interested in having a comparative pathologist assist with designing your animal study, making pathology figures for your manuscript, or writing a grant letter of support? Contact us! CPDISR@cvm.osu.edu.
Meet the team (Back to top)
- Comparative Pathologist: Sue E. Knoblaugh, DVM, Dipl. ACVP
- Medical Laboratory Technologist (Bloodwork): Janae' DuLaney, BS, MLS (ASCP)
- Anatomic Pathology Technologist (Necropsy and Trimming): Julie Rectenwald, AHT, MLT
- Animal Histology Laboratory Manager: Johanna Rawlings
- Histotechnologist: Florina Jaynes, HTL (ASCP)
- Immunohistochemistry Technologist: Kevin Thorburn, PhD, HTL (ASCP), QIHC (ASCP)
- Researcher 1 (Slide Scanning and Customer Service): Raquel Trejo
Please remember to cite the Shared Resources!
Research reported in this publication was supported by The Ohio State University Comprehensive Cancer Center and the National Institutes of Health under grant number P30 CA016058.
We thank the XX Shared Resource at The Ohio State University Comprehensive Cancer Center, Columbus, OH for (XX)